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P2921
Sigma
Monoclonal Anti-Protein A antibody produced in mouse
clone SPA-27, ascites fluid
| Synonym: | Monoclonal Anti-Protein A |
Description
| Immunogen | protein A from Cowan I strain of Staphylococcus aureus. |
| Specificity | Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A. |
Properties
| antibody form | ascites fluid |
| clone | SPA-27 (monoclonal) |
| contains | 15 mM sodium azide |
| application(s) | indirect ELISA: 1:5,000 |
| isotype | IgG1 |
| suitability | suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) |
| suitable for immunohistochemistry (frozen sections) | |
| suitable for immunoprecipitation | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Hahn, R., et al., Comparison of protein A affinity sorbents III. Life time study J. Chromatogr. A. 1102, 224-231, (2006) Abstract |
| Armbruster, C., et al., A simple and robust method for the complete dissociation of HIV-1 p24 and other antigens from immune complexes in serum and plasma samples J. Immunol. Methods 217, 143-151, (1998) Abstract | |
| Armbruster, C., et al., A simple method to quantify staphylococcal protein A in the presence of human or animal IgG in various samples. J. Immunol. Methods 235, 61-69, (2000) Abstract |




