P2921

Sigma

 

Monoclonal Anti-Protein A antibody produced in mouse

clone SPA-27, ascites fluid

Synonym:Monoclonal Anti-Protein A

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Description

Immunogenprotein A from Cowan I strain of Staphy­lococcus aureus.
SpecificitySpecific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A.

Properties

antibody formascites fluid
cloneSPA-27 (monoclonal)
contains15 mM sodium azide
application(s)indirect ELISA: 1:5,000
isotypeIgG1
suitabilitysuitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections)
 suitable for immunohistochemistry (frozen sections)
 suitable for immunoprecipitation
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3

References

referenceHahn, R., et al., Comparison of protein A affinity sorbents III. Life time study J. Chromatogr. A. 1102, 224-231, (2006) Abstract
 Armbruster, C., et al., A simple and robust method for the complete dissociation of HIV-1 p24 and other antigens from immune complexes in serum and plasma samples J. Immunol. Methods 217, 143-151, (1998) Abstract
 Armbruster, C., et al., A simple method to quantify staphylococcal protein A in the presence of human or animal IgG in various samples. J. Immunol. Methods 235, 61-69, (2000) Abstract